Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Aug 27;124(2):278–290. doi: 10.1093/toxsci/kfr225

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2011. Published by Oxford University Press on behalf of the Society of Toxicology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

PMC Copyright notice

FIG. 1. — Hepatocyte-like characteristics of hES-Hep. (A) Phase contrast microscopy demonstrating the typical hES-Hep morphology after 22 days of differentiation. (B) Cells were positive for hepatocyte markers, such as α1-AT, AFP, albumin, CK18, HNF4α, and CYP1A2. (C) qPCR gene expression of hES-Hep show an expression of CYP1A2, CYP3A4, CYP2C9, OCT1, OATP2, GSTA1, and BCEP in comparison with HepG2 and undifferentiated hESC. Y-axis shows log₂ values relative to HepG2 and hESC (n = 4). (D) CYP activity of CYP1A2, CYP3A4, CYP2C9, CYP2C19, and CYP2B6 further supporting that these cells are hepatocyte-like. Y-axis scale is Pmoles total log₂ (n = 3). hpHep = human primary hepatocytes cultured for 48 h. (E) Significance of gene expression of hepatic markers in untreated hES-Hep. Y-axis displays the negative log₁₀ of the detection p values of the gene expression signals. Horizontal lines indicate the 0.1 (orange) and 0.01 (red) detection levels.