. 2004 Feb;72(2):766–773. doi: 10.1128/IAI.72.2.766-773.2004

TABLE 1.

Oligonucleotide primers used in this study

Gene	Primer	Sequence (5′→3′)^a
ureA	UreA-F1	ATGAAACTCACCCCAAAAGA
	UreA-R-T7^b	ctaatacgactcactatagggagaGGAAGTGTGAGCCGATTTGA
amiE	Amid-F1	AGTAGCAGCCCAGATACTGT
	Amid-R-T7^b	ctaatacgactcactatagggagaGCACGATCTCACCCTTATCA
amiF	Form-F1	TCAGTTTCCTGTGCCAATTGTCA
	Form-R-T7^b	ctaatacgactcactatagggagaCTCAATGGGATTCCATGGGAATA
hspA	HspA-F1	CCAGTTCAGGCATCATCATC
	HspA-R-T7^b	ctaatacgactcactatagggagaCAAGAGCCTGAGCCCACAAT
fur	Fur-F1	TCCATTTTAGAGCGCTTGAG
	Fur-R-T7^b	ctaatacgactcactatagggagaTTAACGACTTCATTCTGGCGGTT
nikR	NikR-F1	CACCCAATAAAGACGATTCA
	NikR-R-T7^b	ctaatacgactcactatagggagaAGCTAGACGCCTTAGTCAAT

Primer sequences were derived from the H. pylori 26695 genome sequence (37).

Primers contained a 5′ extension with T7 promoter sequence (in lowercase letters) for the creation of an antisense RNA probe (38).