TABLE 2.
Proliferative responses of CD4+ T-cell lines from B. bovis Hsp20-immunized calves to B. bovis and B. bigemina
| Antigen | Proliferation (cpm) of short-term T-cell linesa from calfb:
|
|||
|---|---|---|---|---|
| 50 (8/1) | 56 (10/7) | 57 (22/11) | 58 (24/12) | |
| Medium | 1,576 ± 210 | 1,666 ± 867 | 463 ± 128 | 113 ± 10 |
| URBC | 953 ± 136 | 1,465 ± 82 | 436 ± 139 | 95 ± 17 |
| B. bovis CM | 62,952 ± 658 | 32,215 ± 1,195 | 11,942 ± 1,094 | 24,922 ± 1,024 |
| B. bigemina CM | 60,068 ± 1,285 | 27,339 ± 1,683 | 10,987 ± 758 | 22,854 ± 1,073 |
a
Short term T-cell lines were established by stimulation with B. bovis CM for 1 week and resting for 1 week. Lymphocytes were cultured for 3 days with 25 μg of the indicated antigens per ml, radiolabeled, and counted. Results are expressed as the mean counts per minute (cpm) of [3H]thymidine incorporation ± 1 SD. Significant proliferation is indicated in bold type.
b
DRB3 haplotypes determined by RFLP-PCR are indicated for each calf.