TABLE 3.
Proliferative responses of CD4+ T-cell lines from B. bovis Hsp20-immunized calves to Hsp20 peptides
| Peptide | Proliferation (SI) by T-cell lines from calfa:
|
|||
|---|---|---|---|---|
| 50 | 56 | 57 | 58 | |
| P1 | 103.1 | 1.7 | 52.9 | 4.1 |
| P2 | 108.0 | 50.6 | 57.9 | 8.7 |
| P3 | 117.0 | 1.1 | 78.8 | 27.1 |
| P4 | 1.4 | 4.9 | 1.6 | 2.4 |
| P5 | 2.3 | 1.9 | 14.7 | 1.3 |
| P6 | 104.2 | 1.4 | 1.5 | 5.1 |
| P7 | 65.3 | 1.7 | 2.8 | 145.7 |
| P8 | 6.5 | 1.7 | 4.4 | 2.4 |
| P9 | 1.5 | 1.4 | 2.7 | 68.1 |
a
PBMC were stimulated for 1 week with B. bovis CM and rested for 1 week before being tested. Results were obtained for the optimal response to 1 or 10 μg of each peptide per ml. SI indicates stimulation indices (mean counts per minute of the response to Hsp20 peptide/mean of the response to control A. marginale MSP2 peptide). Significant responses (SI ≥ 3.0) are indicated in bold type. These responses were significant when individual counts per minute were compared by the one-tailed Student t test (P < 0.005).