Fig. 4.

Aldosterone impairs insulin secretion via an MR-independent mechanism and oxidative stress. a Eplerenone (EPL) did not prevent the effect of aldosterone (Aldo) on insulin secretion in isolated islet static culture. b The water-soluble MR antagonist RU-28318 did not prevent the effect of aldosterone in the clonal MIN6 cell line (0 mmol/l glucose, white bars; 23 mmol/l glucose, grey bars). c MR protein abundance was markedly reduced in MIN6 cells compared with renal cortex. d The antioxidant tempol prevented the effect of aldosterone in isolated islet studies. *p<0.05 and **p≤0.01 vs control