Figure 4. Notch activation in response to odor is Delta dependent.

A–D. N-GV UAS.dGFP/+ flies that are genotypically +/+, Dl^RF/+, Dl^6B/+, or Dl^RF/Dl^6B, were raised at 18°C on molasses food and shifted to dextrose food upon eclosion. Flies were aged at 18°C for 5–6 days and then shifted to 30° C for one day prior to exposure to 5% CO₂ on agar at 30° C for three days. The dGFP response is reduced progressively in the weaker Dl^RF/+ and stronger Dl^6B/+ genotypes, and is absent in Dl^RF/Dl^6B transheterozygotes. See also Fig. S2.

E–H. N-GV UAS.dGFP/+ flies that are genotypically +/+ (E,F) or Dl^RF/Dl^6B (G,H) were raised and aged as in A–D. Flies were then shifted to 30°C for 4 days, shifted back to 18°C for one day and exposed to 5% CO₂ on agar at 18°C for 3 days. The dGFP response is restored upon return to the permissive temperature.

I. The graph presents an experiment analogous to that shown in A–D, except the flies were exposed to a 1:1000 dilution of geranyl acetate, rather than 5% CO₂. The box plots depict dGFP accumulation in the VA6 glomerulus. The results are the same as for CO₂, except that there is no significant decline in the geranyl acetate response in the weaker Dl^RF/+ genotype. The number of samples analyzed was: wt, 20; Dl^RF/+, 22; Dl^6B/+, 14; Dl^RF/Dl^6B, 16.