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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: Neuron. 2011 Feb 10;69(3):468–481. doi: 10.1016/j.neuron.2010.12.015

Figure 5. Notch activation in response to odor requires odorant receptor.

Figure 5

A,B. wild type (A) and Or43b− (B) flies, that are also N-GV/UAS.dGFP, were raised and maintained on dextrose food for three to five days and then exposed to a 1:100 dilution of ethyl butyrate for 4 days. Note the absence of the ethyl butyrate response in VM2 in Or43b mutants, as well as the increase in dGFP accumulation in VA6, DM5, DM6 and D compared to the wild type control.

C. Or43b− flies (C) flies, that are also N-GV/UAS.dGFP, were exposed to a 1:100 dilution of geranyl acetate. Predominant dGFP accumulation is in VA6, as in control (wild type) flies (Fig. 2A).

D–F. Or43b− flies carrying the Or43b.Or82a transgene (Or43b−; 43b>Or82a), as well as N-GV and UAS.dGFP, were exposed to paraffin oil (D), or to 1:100 dilutions of ethyl butyrate (E) or geranyl acetate (F). Ectopic expression of the Or82a receptor in Or43b ORNs innervating VM2 is associated with an ectopic dGFP response to geranyl acetate in VM2 (F), but no rescue of dGFP accumulation in VM2 in response to ethyl butyrate (E). Similarly, it is associated with the loss of the prominent dGFP response seen in VA6 and DM6 in Or43b mutant (B), but not wild type flies (A).