Figure 3. Analyses of FEAT transgenic mice.

(a) FEAT protein expression in nontransgenic (left panel) or transgenic (right panel) mouse organs. Similar results were obtained with first generation offspring of three distinct founders. The blot (30 µg protein/lane) was probed with anti-FEATΔN (upper panels) and anti-actin (lower panels) antibodies. (b) Cell death of thymocytes is attenuated in FEAT transgenic mice. Survival of thymocytes isolated from transgenic mice (Tg (+)) (red, solid lines) or nontransgenic littermates (Tg (-)) (blue, dashed lines) were analyzed by flow cytometry (means ± s.e.m.) after treatment for 24 h with Fas ligand (left panel) (*, P = 0.02492; **, P = 0.0497; ***, P = 0.0226, n = 5; paired t-test) or dexamethasone (right panel) (*, P = 0.0331; **, P = 0.0411, n = 6; paired t-test). (c) Tumours in founder, first and second generation FEAT transgenic mice. HCC, hepatocellular carcinoma.