Table 2. Validation of proteome data using quantitative PCR.

		RT- PCR				Proteomics
		15 min	30 min	60 min	90 min	15 min	30 min	60 min	90 min
Branched Chain Amino Acid Degradation
3-ketoacyl-CoA thiolase	Sama0031	−0.4	0.9	1.9	3.3	−0.5	0.1	1.4	1.2
Hydroxymethylglutaryl-CoA lyase	Sama1358	−1.7	0.7	3.6	1.8	−2.1	2.9	3.9	4.1
3-methylcrotonoyl-CoA carboxylase	Sama1359	−0.5	1.8	2.1	1.8	−0.3	2.1	2.2	2.3
Short chain enoyl-CoA hydratase	Sama1378		0.2	2.2	1.3	0.1	0.5	1.3	1.4
TCA cycle
Acetyl-coenzyme A synthetase	Sama2079	−0.1	2.2	3.1	2.8	−0.4	3.7	4.8	5.1
2-methylcitrate synthase	Sama3295	−0.4	1.1	−0.3	1.6	0.1	1.8	2.0	1.8
Aconitase	Sama3296	1.7	5.8	1.6	3.2	−0.2	1.6	1.8	1.7
Motility
Flagellar biosynthetic protein FlhB	Sama2284	−0.3	−0.5	−1.9	−4.5	nd	nd	nd	nd
Flagellar hook-length control protein FliK	Sama2292	−5.9	−7.9	−1.0	−3.9	nd	nd	nd	nd
Flagellar hook protein FlgE	Sama2315	0.1	−3.0	−0.8	−0.2	nd	nd	nd	nd
Replication and Division
Cell division protein FtsZ	Sama0358	−1.6	−2.1	−0.5	−0.6	−0.2	−0.2	−0.4	−0.2
DNA-directed DNA polymerase	Sama1310	−2.3	−5.3	−1.2	−6.2	−6.1	1.0	−7.0	3.0
DNA topoisomerase IV subunit A	Sama3041	−0.2	−1.7	0.1	−0.8	−0.3	−0.3	−2.1	−0.3

*nd – Not determined