Table 1. Primary antibodies used in this study.
Antibodies | Source | Dilution | Tissues/Cells* |
---|---|---|---|
IgA (alpha), (rabbit Ab) | DAKO | 1/2000 | 1, 2, 3 |
IgG (gamma), (rabbit Ab) | DAKO | 1/1000 | 1, 2, 3 |
IgM (my, µ), (rabbit Ab) | DAKO | 1/1000 | 1, 2, 3 |
Bcl2 (mouse Ab) | DAKO | 1/100 | 2, 4, 5, 6, 7 |
α Smooth Muscle Actin (mouse Ab) | DAKO | 1/50 | 4, 5, 6, 7, 8, 9 |
α Smooth Muscle Actin (rabbit Ab) | AbCam | 1/200 | 4, 5, 6, 7, 8, 9 |
ApoE (rabbit Ab) | Santa Cruz | 1/100 | 10 |
CD3 (mouse Ab | Novocastra | 1/200 | 2, 23 |
CD10 (mouse Ab) | Novocastra | 1/50 | 5 |
CD20 (mouse Ab | DAKO | 1/500 | 2, 3, 10 |
CD32 (mouse Ab) | AbCam | 1/1000 | 3, 23 |
CD34 (mouse Ab) | Novocastra | 1/50 | 1, 9, 25 |
CD61 (mouse Ab) | Novocastra | 1/100 | 10 |
CD68 (mouse Ab) | DAKO | 1/200 | 2, 3, 10 |
CD117, c-Kit (rabbit Ab) | DAKO | 1/100 | 4, 5 |
Cytokeratins 5 (rabbit Ab) | Medac | 1/100 | 4, 5, 6, 7, 8, 24, 25 |
Cytokeratin 5/6 (mouse Ab) | DAKO | 1/50 | 4, 5, 6, 7, 8, 24, 25 |
Cytokeratin 7 (mouse Ab) | DAKO | 1/200 | 4, 5, 6, 7, 8, 24, 25 |
Cytokeratin 10 (mouse Ab) | DAKO | 1/50 | 4, 5, 6, 7, 13 |
Cytokeratin 14 (mouse Ab) | Jackson ImmunoRes | 1/500 | 4, 5, 6, 7, 8, 24, 25 |
Cytokeratin 18 (mouse Ab) | Sigma | 1/50 | 4, 5, 6, 7, 8, 24 |
Cytokeratin 8/18 (mouse Ab) | Zytomed | 1/50 | 4, 5, 6, 7, 8, 24, 25 |
Cytokeratin AE1/AE3 (mouse Ab) | DAKO | 1/50 | 2, 4, 5, 6, 7, 13 |
Cytokeratins 8/18/19 (mouse Ab) | Immunotech | 1/100 | 4, 5, 6, 7, 8, 25 |
Collagen IV (mouse Ab) | DAKO | 1/20 | 5, 11 |
Desmin (mouse Ab) | DAKO | 1/200 | 12, 14 |
E-Cadherin (mouse Ab) | DAKO | 1/50 | 1, 4, 5 |
Calcitonin (rabbit Ab) | DAKO | 1∶500 | 15 |
Calponin (mouse Ab) | DAKO | 1/50 | 9, 16, 17 |
EMA (mouse Ab) | DAKO | 1/50 | 1 |
Estrogen Receptors (rabbit Ab) | Thermo | 1/200 | 4, 5 |
GFAP (mouse Ab) | DAKO | 1/100 | 18 |
GFP (rabbit Ab) | AbCam | 1/500 | 19 |
HMB45 (mouse Ab) | DAKO | 1/50 | 13 |
Kappa Light Chains (rabbit Ab) | DAKO | 1/8000 | 2, 10 |
Lambda Light Chains (rabbit Ab) | DAKO | 1/8000 | 2, 10 |
Ki67 (rabbit Ab) | Thermo | 1/200 | 2, 4, 5, 6 , 7, 24 |
MIB1 (mouse Ab) | DAKO | 1/20 | 2, 4, 5, 6 , 7, 24 |
Myf-4 (mouse Ab) | Zytomed | 1∶50 | 20 |
nNOS (rabbit Ab) | Transduction Lab. | 16, 17, 21, 22 | |
eNOS (rabbit Ab) | Transduction Lab. | 9, 16, 17, 21, 22 | |
P53 (mouse Ab) | DAKO | 1/50 | 5, 7 |
p63 (mouse Ab) | DAKO | 1/200 | 4, 5, 6, 7 |
S100 (rabbit Ab) | DAKO | 1/2000 | 2, 5, 7, 10 |
Vimentin (mouse Ab) | DAKO | 1/200 | 15 |
Vimentin (rabbit Ab) | AbCam | 1/1000 | 5, 9 |
*Cell and tissue samples immunostained in this study: Human kidney (1), Human tonsil (2), Human lymph nodes (3), Human mammary gland (4), human breast tumors (5), Human salivary gland (6), Human salivary gland tumors (7), Human lacrimary gland (8), Human aorta (9), Human bone marrow (10), Human bone tissue (11), Human gastrointestinal tissue (12), Human skin (13), neonatal-rat-cultured cardiomyocytes (14), Human thyroid gland (15), Human muscle tissue (16), Rat muscle tissue (17), Human brain astrocytoma (18), Mouse heart, spleen and kidney (19), Human rabdomyosarcoma (20), Human pancreas (21), Rat pancreas (22), Human blood cell smears and cytospins (23), Cell cultures of human adenoid cystic carcinoma (24), Cow and pig mammary gland (25)