TABLE 1.

Detection of flavivirus antibodies by the WNV-E MIA and by ELISA in a blinded serum panel

Serum no.	Etiologic virus, PRN titer	P/N ratioa
		rWNV-E MIA, polyvalent	WN virus ELISA, IgG, NYSb	WN virus ELISA, IgG, CDC	WN virus ELISA, IgM, NYS	WN virus ELISA, IgM, CDC	DEN virus ELISA, IgG, NYS	SLE virus ELISA, IgG, CDC	SLE virus ELISA, IgM, CDC
1	NEGc	1.31	2.01	1.20	1.51	1.59	0.12	NDd	ND
2	NEG	0.79	0.78	ND	0.93	1.17	0.08	ND	ND
3	NEG	0.81	0.62	ND	0.96	0.95	0.10	ND	ND
4	NEG	1.90	0.48	0.82	11.79	5.04	0.16	ND	ND
5	NEG	0.86	0.96	0.89	0.46	1.26	0.14	ND	ND
6	NEG	2.62	1.06	0.97	5.89	2.23	0.31	ND	ND
7	NEG	1.48	0.88	0.90	1.34	1.25	0.30	ND	ND
8	WN virus, 160	5.89	8.96	4.40	5.76	4.02	0.49	ND	ND
9	WN virus, 160	45.15	13.96	5.28	16.25	8.90	2.58	ND	ND
10	WN virus, 320	42.99	12.77	5.80	13.73	6.16	3.04	ND	ND
11	SLE virus, 2,560	4.28	2.56	ND	7.57	3.26	0.57	1.68	8.89
12	SLE virus, 40	0.90	1.44	1.05	1.98	1.52	0.10	1.39	4.48
13	SLE virus, 1,280	18.88	7.26	7.05	10.06	3.67	1.34	7.69	8.53
14	SLE virus, 1,280	14.34	3.17	3.63	14.33	7.00	0.86	5.45	8.69
15	SLE virus, 80	4.80	1.43	1.45	8.44	3.65	0.19	ND	8.43
16	SLE virus, 10	0.80	0.98	0.77	3.52	1.74	0.20	0.80	2.76
17	DEN virus, ND	49.90	20.06	ND	12.90	1.62	10.39	ND	ND
18	DEN virus, ND	15.99	3.22	ND	13.59	1.72	1.59	ND	ND
19	DEN virus, 160	55.23	15.85	ND	3.85	ND	8.28	ND	ND

^aBoldface indicates positive result (P/N ratio was greater than the cutoff value).

^bNYS, tests were performed at the New York State Department of Health, Wadsworth Center, Albany, N.Y.

^cNEG, specimen was negative to neutralizing flavivirus antibodies.

^dND, test was not performed on specimen.