Table 1. Assay protocols and DMR signals used for similarity and correlation analysis.

Assay number	Probe, pretreatment duration	DMR readout	Note
1	0.1% DMSO, 1hr	Ligand, 10 μM	Positive control, agonism
2	10 μM Ligand, 1hr	Epinephrine, 5 nM	Specificity, relative potency and efficacy, modes of action
3	1 μM propranolol, 1hr	Ligand, 10 μM	Specificity, relative potency and efficacy, modes of action
4	5 nM Epinephrine, 1hr	Ligand, 10 μM	Receptor desensitization and deactivation
5	10 μM ligand, 5hr	Epinephrine, 5nM	Kinetics dependent receptor blockage by antagonists and receptor desensitization by agonists
6	5 nM Epinephrine, 5hr	Ligand, 10 μM	Kinetics dependent deactivation of the receptor after prolonged activation with epinephrine
7	10 μM ligand, 5hr	Propranolol, 10 μM	Relative potency, kinetics dependent agonism
8	0.1% DMSO, 1hr	Forskolin 10 μM	cAMP-PKA pathway
		Ligand, 10 μM
9	400 ng/ ml cholera toxin, 20hr	Ligand, 10 μM	Gαs-dependent and independent activity
10	100 ng/ml pertussis toxin, 20hr	Ligand, 10 μM	Gαi sensitivity
11	10 μM TBB, 1hr	Ligand, 10 μM	CK2 sensitivity
12	10 μM U0126, 1hr	Ligand, 10 μM	MEK1/2 sensitivity