Table 1. Seizure Indices (Si).
| Genotype | n | % seizedŦ | Avg. Ts±sd (°C)* | Avg. Amax±sd (mV)* | Avg. Area±sd (pixel × 10-3)* | Median Si** (25th/75th percentiles) | +P-value |
|---|---|---|---|---|---|---|---|
| OregonR | 7 | 29 | 40.7±6.1 | 12.2±13.4 | 2.9 ± 2.4 | 0.0 (0.0/0.9) | 0.944 (χ2 = 0.115) |
| w1118;CyO/+; TM6/+ | 4 | 25 | 46.4 | 2.6 | 1.3 | 0.0 (0.0/0.4) | |
| w1118;CyO/Pin | 4 | 25 | 38.3 | 14.3 | 6.9 | 0.0 (0.0/0.6) | |
| wt*** | 15 | 24 | 42.1±4.1 | 14.0±13.8 | 4.5±2.9 | 0.0 (0.0/0.8) | - |
| DysDf/DfKX23 | 5 | 80 | 37.0±3.1 | 6.0±5.2 | 2.4 ± 2.0 | 1.8 (1.7/2.2) | 0.028* |
| DfKX23/+ | 4 | 100 | 43.0±3.3 | 11±6.8 | 4.9 ± 5.4 | 1.8 (1.5/2.2) | 0.011* |
| DysDf/Dys8-2 | 10 | 60 | 40.8±6.4 | 13±6.4 | 4.2 ± 2.9 | 1.6 (0.0/2.2) | 0.045* |
| Dys8-2/+ | 5 | 80 | 40.2±8.2 | 3.3±1.7 | 1.4 ± 0.8 | 1.7 (1.3/1.8) | 0.045* |
| DysDf | 9 | 89 | 35.2±4.7 | 7.7±6.4 | 4.9 ± 3.7 | 2.4 (2.2/2.4) | 2.6 × 10−4*** |
| DysDf/+ | 8 | 75 | 39.6±3.5 | 8.7±5.3 | 2.4 ± 2.5 | 1.7 (1.1/1.8) | 0.050* |
| ◊Dg−/− | 9 | 11 | 45.3 | 5.6 | 1.3 | 0.0 (0.0/0.0) | 0.19 |
| DgO86/+;DysDf/+ | 10 | 20 | 38.0±7.6 | 5.2±2.9 | 2.8 ± 0.3 | 0.0 (0.0/0.0) | 0.50 |
| ♦parats1/+; DysDf/+ | 5 | 0 | 0 | 0 | - | - | 0.28 |
| corak08713 | 8 | 0 | - | - | - | - | 0.15 |
| corak08713/+; DysDf/+ | 8 | 12.5 | 36.0°C | 5.2 | 5.6 | 0.0 (0.0/0.0) | 0.41 |
| ♦Camn339/+; DysDf/+ | 7 | 0 | - | - | - | - | 0.19 |
| tub-Gal4/+ | 10 | 20 | 42.5±5.5 | 2.6±0.1 | 1.6±0.4 | 0.0 (0.0/0.0) | - |
| tub-Gal4::dsDys/+ | 10 | 70 | 40.1±5.8 | 4.5±2.1 | 2.3 ±1.8 | 1.7 (0.2/1.9) | 0.020* |
| tub-Gal4::dsDg/+ | 8 | 50 | 43.6±2.6 | 4.1±2.1 | 1.2 ±0.7 | 0.7 (0.0/1.6) | 0.19 |
| tub-Gal4::dsDys/dsDg £ | 6 | 50 | 44.2±5.3 | 12±13.4 | 0.8 ±0.2 | 0.7 (0.0/1.4) | 0.29 |
| tub-Gal80ts/+, tub-Gal4::dsDys/+ – Put to 29°C after eclosion | 14 | 29 | 45.7±2.4 | 10±5.8 | 2.1 ± 1.2 | 0.0 (0.0/0.9) | 0.47 |
| tub-Gal80ts/+, tub-Gal4::dsDys/+ – Put to 29°C as L3 larvae | 13 | 77 | 40.1±4.9 | 7.9±5.2 | 4.2 ± 4.2 | 2.0 (1.7/2.2) | 3.7 × 10−4*** |
| tub-Gal80ts/+, tub-Gal4::dsDg/+ – Put to 29°C after eclosion | 5 | 0 | - | - | - | - | 0.40 |
| tub-Gal80ts/+, tub-Gal4::dsDys/dsDg – Put to 29°C after eclosion | 6 | 17 | 48.2 | 5.1 | 0.4 | 0.0 (0.0/0.0) | 0.34 |
| parats1/+; tub-Gal4::dsDys/+ | 5 | 0 | 0 | 0 | - | - | 0.40 |
| CyO(Pin)/+;24B-Gal4/+ | 4 | 0 | - | - | - | - | - |
| dsDys/24B-Gal4 | 5 | 100 | 41.5±6.3 | 5.8±4.1 | 3.1 ± 2.7 | 1.8 (1.7/1.8) | 0.0079** |
| dsDg/24B-Gal4 | 5 | 0 | - | - | - | - | 1.0 |
| D42-Gal4/+ | 4 | 0 | - | - | - | - | - |
| dsDys/D42-Gal4 | 5 | 40 | 37.8±8.2 | 5.7±5.2 | 2.3 ± 0.8 | 0.0 (0.0/1.8) | 0.28 |
| dsDg/D42-Gal4 | 5 | 0 | - | - | - | - | 1.0 |
n = number of animals measured for the specified genotype
Ŧpercent of animals measured that had a seizure
*Calculated using data from animals that seized only
**Index calculated by integrating the area of the graph of voltage vs. temperature during a seizure, taking the natural logarithm of this number, dividing by the temperature that the seizure started, and then multiplying times ten. A Si of zero was assigned to animals that did not seize
***There is no difference between animals of genotypes OregonR, w1118;CyO/+;TM6/+ or w1118;CyO/Pin as assessed using a Kruskal-Wallis test. These animals were pooled and termed WT.
+P-values are relative to the control animals at the beginning of each shaded section, *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001
◊Five animals were of genotype DgO86/O55, two were of genotype DgO86 and two were of genotype DgO86/+ where one animal of genotype DgO86/O55 had a seizure
£Four animals were of genotype tub-Gal4::dsDg/dsDys and two animals were of genotype tub-Gal4::dsDys/dsDg
♦Animals of genotype parats1/Y and Camn339/+ did not have seizures (n = 2)