Figure 3. Cloning and analysis of the predicted miRNA by stem-loop PCR and northern blot.

Stem-loop PCR was performed to clone and analyze the proper expression of the predicted miRNAs in (A) HzNV-1 productively infected cells, (B) pag1-transfected cells, and (C) HzNV-1 latently infected cells. (D, E) Predicted secondary structures of hv-miR-246 5P (D), and hv-miR-2959 5p (E), precursors. (F) Small RNAs harvested from HzNV-1 productively infected cells at various time points were analysis by northern blots with probes against predicted HzNV-1 miRNAs (top panels) or let-7a miRNA as a positive control (bottom panels).