Figure 2. Expression of cellular senescence-related markers 53BP1 and γ-H2AX in BMSCs isolated from young mice (1–2 months old, n = 3) and aged female Balb/C mice (20–24 months old, n = 3).

(A) 53BP1 expression (green) in BMSCs nuclei overlapping with DAPI staining (blue). (B) γ-H2AX expression (green) in BMSCs nuclei overlapping with DAPI staining (blue). (C) Percentage of 53BP1 and γ-H2AX positive cells in BMSCs. *: p = 0.014, **: p = 1.71E-4.