TABLE 2.

PCR primer sets for detection of superantigenic genes and streptolysin S gene (sagA)

Gene	Primer	Primer sequence	Reference
speA	speA-F	5′-CAACAAGACCCCGATCCA-3′	50
	speA-R	5′-CTTGGTTGTTAGGTAGAC-3′
speB	speB-F	5′-GGCGATTTCAGAATTGATGGC-3′	7
	speB-R	5′-TAGTGCGTCAAGACGGAAG-3′
speC	speC-F	5′-CCGAAATGTCTTATGAGGCC-3′	7
	speC-R	5′-CCCTTCATTTGGTGAGTC-3′
speF	speF-F	5′-ATGAATCTACTTGGATCAAG-3′	This study
	speF-R	5′-TTATTTCTGAGTAGGTGTAC-3′
spegg	spegg-F	5′-ATGAAAACAAACATTTTGACAATTATC-3′	This study
	spegg-R	5′-CTAGTGCGTTTTTAAGTAGATA-3′
speH	speH-F	5′-AATTCTTATAATACAACCAATAGAC-3′	37
	speH-R	5′-TTAGCTGATTGACACATCTA-3′
speI	speI-F	5′-ATGAGTAGTGTGGGAGTTATTAA-3′	36
	speI-R	5′-TTTAGCATACTCTCTCTGTCAC-3′
speJ	speJ-F	5′-GATAGTGAAAATATTAAAGACG-3′	36
	speJ-R	5′-TTATTTAGTCCAAAGGTAAATATC-3′
speL	speL-F	5′-GTCATATCATGTTGTATGCAA-3′	21
	speL-R	5′-GTTTAAGTGAACATCAAAGTG-3′
mf-2	mf2-F	5′-CTAGGGTACGAACTTATCC-3′	16
	mf2-R	5′-TTAGTTTTTAGGAGTGGCAG-3′
mf-3	mf3-F	5′-GCCAGAAAAATTAGAAATTT-3′	16
	mf3-R	5′-TTAAGCCGCTTCTTCAAACT-3′
smeZ	smeZ-F	5′-TTAGAAGTAGATAATAATTCCCTT-3′	37
	smeZ-R	5′-TTAGGAGTCAATTTCTATATCTAAA-3′
sagA	sagA-F	5′-TTACTTCAAATATTTTAGCTACTAG-3′	This study
	sagA-R	5′-TTATTTACCTGGTGTATAGC-3′