Figure 5. Oxysterols induce apoptosis in endothelial cells and astrocytes in vitro.

Western Blot analysis shows the cleavage of Caspase-3 induced by β−epoxycholesterol and 7-ketocholesterol in Bovine Aortic Endothelial cells (A) and C8-D1A astrocytes (B). Staurosporin (ST) was used as positive control. Actin level is reported to normalize the quantity of protein in each sample (n = 3, n = number of experiments) (Bonferroni corrected t-test: *P <0.01).