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. 2011 Sep 14;1:92. doi: 10.1038/srep00092

Figure 1. Delineation of the nuclear localization signal (NLS) of Y14.

Figure 1

(A) HeLa cells were transfected with expression vectors encoding myc-tagged pyruvate kinase (PK) fusion proteins. The numbers above each panel correspond to the aa within Y14 that were fused to PK. The subcellular localization of each protein was determined by indirect immunofluorescence using anti-myc monoclonal antibody, 9E10. (B) Schematic drawing of the PK fusion proteins and summary of their intracellular localization as displayed in (A). Also shown is the sequence of the minimally defined Y14 NLS.