Figure 3. C-terminal of Y14 is also required for binding to Magoh in addition to the RNA-binding domain.

Wild-type and mutant myc-tagged Y14 proteins, produced and labeled with [35S]methionine in vitro, were incubated with 5 μg each of GST or GST-Magoh. After washing, bound proteins were eluted, separated on 15% SDS-PAGE gel and visualized by fluorography. The lanes marked Input contain 10% of the total protein used in each binding reaction. The position of molecular mass markers is shown on the left.