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. 2011 Sep 14;1:92. doi: 10.1038/srep00092

Figure 4. Y14 binding to spliced mRNA is Magoh-independent and requires its C-terminus.

Figure 4

(A) Preparation of various recombinant Y14 proteins in E. coli. Wild-type and three mutant proteins were expressed in E. coli cells and purified using Ni-NTA column. Five hundred ng of each purified protein was analyzed by SDS-PAGE. (B) Immunoprecipitation of RNAs from in vitro splicing reaction with Ade2 pre-mRNA in the presence of recombinant proteins was performed. 2.5 μg of anti-T7 tag antibody was used for immunoprecipitation. Pre-mRNAs and mRNA products are indicated with schematic drawings on the right. (C) RNA immunoprecipitation experiment with chicken δ-crystallin pre-mRNA was carried out as in (B).