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. 2011 Sep 19;1:95. doi: 10.1038/srep00095

Figure 2. Specificity of Dm-ChP procedure.

Figure 2

(A) Dm-ChP was performed in the presence (+) or absence (−) of the indicated components. Input and Dm-ChP material was analyzed by western blotting using anti-histone H3 antibodies. (B) Dm-ChP was performed with (+ EdU) or without EdU labeling in cells either expressing GFP-histone H3 (HeLa GFP-H3) fusion protein or parental HeLa cells (HeLa). 600 μg of extract from the indicated samples (+) was either directly used for Dm-ChP or mixed together before streptavidin pull-down. Input (25 μg) and Dm-ChP material was analysed by western blotting using anti-GFP antibody (top panel), anti-histone H3 (middle panel) or anti-histone H4 (bottom panel) antibodies.