Figure 3. Detergent screening for optimal solubilization of hTAAR5 (a) and hTAAR5-T4L (b) expressed in HEK293S cells.

Expression of hTAAR5 or hTAAR5-T4L was induced with tetracycline (1μg/ml) and sodium butyrate (2.5mM) for 48h, and receptors were solubilized in PBS containing detergents for 1 hour at 4°C. In total, 96 detergents or detergent-mixtures were screened. Each integer from 1 to 96 along the x-axis represents a detergent or detergent mixture (see Supplementary Table S1 online for more information about each detergent or detergent mixture). The ability of each detergent or mixtures to solubilize hTAAR5 or hTAAR5-T4L was been quantified by spot densitometry and normalized to the range from 0.0 to 1.0 for ease of comparison.