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. 2011 Sep 28;1:102. doi: 10.1038/srep00102

Figure 4. Immunoaffinity purification of hTAAR5-T4L. Induced HEK293S cells were harvested and solubilized.

Figure 4

The expressed protein was captured by sepharose beads linked to the anti-rho-tag monoclonal antibody. The captured proteins were washed and then eluted using the rho-tag elution peptide TETSQVAPA. Samples were analyzed on a western blot. Abbreviations: FT, flow through; W, wash; E, elution.