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Detection limit of nested PCR on DBS
| No. of ACH2 cells/spota | Eluate vol for PCR (μl) | No. of HIV proviral copies/PCRb | % Positive |
|---|---|---|---|
| 25 | 10 | 1.25 | 75 |
| 50 | 10 | 2.5 | 83.3 |
| 100 | 10 | 5 | 87 |
| 500 | 10 | 25 | 100 |
Each spot consisted of 50 μl of HIV-negative whole blood spiked with ACH2 cells.
The number of HIV proviral copies per PCR was calculated by the following equation: (number of ACH2 cells per spot/elution volume [200 μl]) × eluate volume used for PCR.
