Table 1. Double-gene knockouts in S. sanguinis.
| Double mutant | Donor | Product Name | Acceptor | CFU | Gel | Essential |
|---|---|---|---|---|---|---|
| Ssx_0791::1494 | SSA_0791 | UDP-N-acetylglucosamine 1-carboxyvinyltransferase | Ssx_1494 | 0 | yes | |
| Ssx_0578::2195 | SSA_0578 | nicotinic acid mononucleotide adenylyltransferase | Ssx_2195 | 0 | yes | |
| Ssx_0352::1188 | SSA_0352 | ribonuclease HIII | Ssx_1188 | 0 | yes | |
| Ssx_2168::1827 | SSA_2168 | NAD(P)H-dependent glycerol-3-phosphate dehydrogenase | Ssx_1827 | >5000 | 1-band | No |
| Ssx_0169::1494 | SSA_0169 | hypothetical protein | Ssx_1494 | >5000 | 1-band | No |
| Ssx_0169::2195 | SSA_0169 | Donor Product Name | Ssx_2195 | >5000 | 1-band | No |
| Ssx_0169::1188 | SSA_0169 | hypothetical protein | Ssx_1188 | >5000 | 1-band | No |
| Ssx_0169::1827 | SSA_0169 | hypothetical protein | Ssx_1827 | >5000 | 1-band | No |
All mutants with double crossover replacement are named Ssx. The number indicates the replaced gene.