Figure 7. Htt co-fractionates with microtubule motors and Rps6 in mouse brain.

(a) Western blot of glycerol gradient fractions for indicated proteins (at right). Increasing numbers correspond to lower (denser) position in the gradient. The material loaded onto the gradient is represented in the input lane (3%). Dhc, dynein heavy chain. Dctn 1, dynactin 1. (b) Proposed model of Htt-mediated RNP transport in the dendrite. Htt/HAP1/dynein/dynactin complex is involved in retrograde transport of RNP, whereas Htt/HAP1/kinesin is involved in anterograde transport of RNP. Exact placement of Htt is not known (indicated by a dashed rectangle).