Figure 3. Temporal context-dependent suppression of RP induction.

(a) The conditioning [Ca²⁺]_i increases used in the simulation. Strong (2 μM for 10 sec)-weak (0.35 μM for 80 sec) or weak-strong [Ca²⁺]_i increase was applied. (b) Simulated time courses of active CaMKII amount before and after the two patterns of coupled [Ca²⁺]_i increase. (c, d) Representative images (c) and time courses (d) of fura-2 fluorescence ratio (F340/F380) before and after the coupled conditioning depolarizations. n = 9 for each. (e, f) Representative current traces (e) and time courses of amplitudes (f) of GABA responses before and after the coupled conditioning depolarizations. n = 5 for each.