Figure 4. Simulation of initial TRAIL signaling model.

(A) Static topology of the TRAIL signaling pathway used in developing our computational model. Note that we lump the similar effects of DR4/5 as TRAILR1/2, and ignore the response of DcR1/R2/OPG. Also, note that we include molecular conditions such as receptor clustering as additional first-order terms. (B) Comparison of simulations (solid lines) with experimental data (dotted lines) in wildtype, RIP1 KD, FADD KD, caspase-8 KD* and TRAF2 KD in arbitrary units (a.u.). The error between simulations and experimental data for the i^th molecule in the k^th condition is calculated based on the area between experimental and simulation curves (see Eq. 5 in Methods). *caspase-8 KD also refers to pro-caspase-8 KD.