FIG. 2.

(a) M. tuberculosis mass spectrum displaying all signals generated by G-specific cleavage of the 16S rDNA amplicon after RNA transcription. To simplify identification, all mass signals corresponding to main cleavage products are marked with numbers. (b) List of detected mass signals corresponding to the spectrum depicted in panel a. For all signals the sequence and location within the uncleaved PCR amplicon is provided in the description column (position follows “@”). MAIN, main cleavage product; LAST, product at the 3′ end of the amplicon, often elongated by one nucleotide as a result of 3′-terminal transferase activity of T7 RNA polymerase.