TABLE 1.
Mycobacterial strains used in this study and typing results of repeated MALDI-TOF MS analysis of clinical isolates
| Species | Source | EMBL accession no. |
|---|---|---|
| Reference strains (n = 12) | ||
| Mycobacterium abscessus | DSM 44196 | AJ536038 |
| Mycobacterium avium subsp. avium | DSM 44156 | AJ536037 |
| Mycobacterium celatum | DSM 44243 | AJ536040 |
| Mycobacterium fortuitum subsp. fortuitum | DSM 46621 | AJ536039 |
| Mycobacterium gordonae | DSM 44160 | AJ536042 |
| Mycobacterium intracellulare | DSM 43223 | AJ536036 |
| Mycobacterium kansasii | DSM 44162 | AJ536035 |
| Mycobacterium marinum | DSM 44344 | AJ536032 |
| Mycobacterium scrofulaceum | DSM 43992 | AJ536034 |
| Mycobacterium smegmatis | DSM 43758 | AJ536041 |
| Mycobacterium tuberculosis | H37RV | AJ536031 |
| Mycobacterium xenopi | DSM 43995 | AJ536033 |
| Clinical isolatesa (n = 24) | ||
| Mycobacterium aurumb | MT1323 | |
| Mycobacterium avium | MT1367 | |
| Mycobacterium avium | MT1951 | |
| Mycobacterium chelonae | MT1297 | |
| Mycobacterium fortuitum | MT2014 | |
| Mycobacterium gordonae | MT804 | |
| Mycobacterium gordonae | MT2843 | |
| Mycobacterium interjectumb | MT1223 | |
| Mycobacterium intracellulare | MT1881 | |
| Mycobacterium intracellulare | MT2544 | |
| Mycobacterium kansasii | MT1619 | |
| Mycobacterium marinum | MT3065 | |
| Mycobacterium paraffinicumb | MT1423 | |
| Mycobacterium smegmatis | MT3344 | |
| Mycobacterium tuberculosis | MT1551 | |
| Mycobacterium tuberculosis | MT1598 | |
| Mycobacterium tuberculosis | MT1692 | |
| Mycobacterium tuberculosis | MT1905 | |
| Mycobacterium tuberculosis | MT2068 | |
| Mycobacterium tuberculosis | MT2125 | |
| Mycobacterium tuberculosis | MT2271 | |
| Mycobacterium tuberculosis | MT2924 | |
| Mycobacterium xenopi | MT309 | |
| Mycobacterium xenopi | MT2236 |
a
Species determined by partial 16S rDNA sequencing and phenotypic methods (M. chelonae, M. kansasii, M. marinum, and M. tuberculosis). All clinical isolates were correctly identified by repeated MALDI-TOF MS (in triplicate) unless otherwise indicated.
b
Correct identification after addition of calculated-mass signal pattern from published 16S rDNA sequences into the database.