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. Author manuscript; available in PMC: 2011 Nov 15.

Published in final edited form as: Biochemistry. 2007 Sep 18;46(40):11389–11397. doi: 10.1021/bi700125e

Table 3.

Effect of the substitutions of unique amino acid residues of hMC2R on ¹²⁵I ACTH binding and cAMP production

	Receptor expression (% of WT)	¹²⁵I- ACTH binding Ki (nM)	cAMP production EC₅₀ (nM)

hMC2RWT	100	8.6 ± 1.0	4.8 ± 0.1
N81A	89 ± 12.4	15.9 ± 0.6^*	11.6 ± 1.2^*
D104N	78 ± 13.4	87.3 ± 9.8^*	87.7 ± 9.3^*
F108A	65 ± 10.2	>10³^*	>10³^*
T164A	93 ± 14.2	6.4 ± 0.7	3.9 ± 0.1
F168A	56 ± 9.5	217.8 ± 13^*	327.6 ± 30.3^*
F244A	95 ± 11.2	9.9 ± 0.9	4.9 ± 0.9

^*

P<0.05 compared with WT receptor