Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Nov 15;22(22):4205–4219. doi: 10.1091/mbc.E11-06-0511

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Subota et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 1: — ALBA protein expression and localization in wild-type and ALBA::YFP–expressing cell lines. (A–C) Reactivity of different ALBA antibodies assessed by Western blot in the indicated cell lines (2 μg of total protein extracts per lane). (A) An antibody recognizing both ALBA3 (21 kDa) and ALBA4 (25 kDa) was used on protein extracts from procyclic form 427 (PCF) cells. (B) A specific anti-ALBA3 antibody and (C) an antibody mostly specific for ALBA4 were assessed in cell lines expressing either ALBA3::YFP (46 kDa) or ALBA4::YFP (50 kDa) fusion proteins in PCF wild-type Lister 427 and in bloodstream-form parasites (BSF) of the strain 9013 (Wirtz et al., 1999). (D) Schematic representation of the procedure used for endogenously tagging ALBA proteins. The plasmid p3329, containing a fragment of the ALBA gene upstream of the YFP sequence and a puromycin-resistance cassette, is linearized (red line) within the ALBA sequence before transfection. This allows homologous recombination with one of the endogenous ALBA copies to create YFP-tagged ALBA. (E) Cultured procyclic cells were analyzed by IFA using the anti-ALBA3 antibody (top) and counterstained with DAPI (white, bottom) merged with the phase contrast image. Cells of the Lister 427 strain expressing (F) endogenously ALBA3::YFP or (G) ALBA4::YFP were fixed with PFA and counterstained with DAPI (white, bottom). Scale bar, 5 μm.