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. 2011 Nov 15;22(22):4205–4219. doi: 10.1091/mbc.E11-06-0511

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© 2011 Subota et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Characterization of the transition from mesocyclic trypomastigote to epimastigote stage in the GFP control and the ALBA3::GFP–expressing strain. Still images extracted from movies of cells of the AnTat1.1 strain expressing GFP control (A) or the ALBA3::GFP (B) after fly dissection. Phase images were superimposed with the fluorescent DAPI signal in blue. Black bars indicate the distance between the nucleus and the kinetoplast. Scale bar, 5 μm. Cellular (C) and nuclear parameters (D) in mesocyclic (MS), parasites in transition from mesocyclic trypomastigote to epimastigote (MS-E), and atypical forms (ATYP, found in ALBA3::GFP infected flies only). Numbers of analyzed parasites are indicated in the bars. (C) Left axis, total cell length plotted as dark gray bars (μm ± SD); right axis, cell diameter shown as red curve (μm ± SD). (D) Left axis, nucleus length plotted as light gray bars (μm ± SD); right axis, distance between the nucleus and the kinetoplast centers shown as blue curve (μm ± SD). Analysis of variance tests were performed, and significant comparisons by Tukey ad hoc posttests are indicated on the histogram with *p < 0.0001.