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. 2011 Nov 15;22(22):4279–4287. doi: 10.1091/mbc.E11-02-0112

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© 2011 Vukajlovic et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 3: — The C-terminal end of the stalk is essential for KLP11/20 dimerization. (A) The N-terminal halves of KLP11 and KLP20 in Figure 2A were elongated in six steps to include increasing numbers of residues from the stalk region. None of these extensions resulted in copurification of the coexpressed partners (Supplemental Figure 2). (B) Dimerization takes place only if the entire stalk region is included as shown schematically on the left panel. The right panel shows the successful copurification of KLP11-6xHis with KLP20-Flag via anti-Flag. (C) An elongation of the RC tails toward the N terminus by merely ten residues is sufficient for copurification via anti-Flag. Numbers correspond to the amino acid positions in the FL chain. The identities of all protein bands were confirmed by mass spectrometry (LC-MS/MS). Marker protein sizes are shown in kilodaltons.