FIGURE 3:

Expression of Msps TOG domains is sufficient to partially rescue Msps microtubule polymerization and EB1 dynamics. (A) (Supplemental Movie S4) Interphase S2 cell expressing EB1::EB1-GFP with wild-type localization (inset, B) and velocity visualized using a 180-s maximum projection (C). Localization and velocity are altered when cells are Msps dsRNA treated (D–F) but can be partially rescued by addition of TOG1-5 TagRFP (G–I), TOG1-4 TagRFP (J–L), TOG1-2 (M–O, and Supplemental Movie S5), and a very small amount by TOG3-4 (P–R). Insets in G, J, M, and P show TagRFP expression. (S) Distribution of EB1 velocities. Dots indicate velocities outside the 10th and 90th percentiles. Numbers in parentheses indicate cells analyzed and number of velocities, respectively. (T) Endogenous EB1 immunofluorescent comet length in cells treated with control or Msps dsRNA. Error bars, 95% CI. Asterisks indicate p value calculated using an unpaired t test between two conditions (*p < 0.05; ***p < 0.0005).