Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2011 Nov 15;22(22):4343–4361. doi: 10.1091/mbc.E11-06-0520

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2011 Currie et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

“ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society of Cell Biology.

PMC Copyright notice

FIGURE 4: — Structure–function analysis of Mini spindles reveals two microtubule lattice–binding sites. (A) Domain structure of Drosophila Dis1/XMAP215 homologue Mini spindles. COOH-terminal TACC interaction domain is indicated as a rectangle. All Msps constructs were COOH-terminally tagged with TagRFP and transfected with GFP–α-tubulin to observe localization to distinct microtubule structures. (B) Msps TOG1-5 TagRFP (left) colocalizes with GFP–α-tubulin (middle) in interphase and spindle poles, spindle microtubules, and condensed chromosomes in mitosis (C). (D) Msps TOG1-4 TagRFP (left) does not colocalize with GFP–α-tubulin (middle) in interphase or mitosis but does localize to condensed chromosomes in mitosis (E). Insets represent single TagRFP channel from merge. (F) Linker4-TOG5 TagRFP in interphase (left) or mitotic (right) S2 cells. (G) Linker2-TOG3 in interphase (left) and mitotic (right) S2 cells.