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. 2011 Nov 15;22(22):4343–4361. doi: 10.1091/mbc.E11-06-0520

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© 2011 Currie et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 5: — Msps has a novel microtubule lattice–binding site that spans the linker region between TOGs 4 and 5 and TOG5 itself. (A) Detailed domain structure of TOG4–TOG5 (821–1428). Interphase (top) and mitotic (bottom) localization of linker4-TOG5 F1204E TagRFP (B), linker4-TOG5 charge reversal TagRFP (residues 1102–1107) (C), chimeric linker4-TOG1 TagRFP (D), and linker4 TagRFP (residues 1079–1173) (E). (F) linker2-TOG3 TagRFP with KVLK charge-reversal mutation in interphase (left) and mitosis (right). Scale bars, 10 μm. (G) Representative Western blot from one in vitro microtubule cosedimentation assay replicate. (H) Binding curves summarizing in vitro cosedimentation assays for various Msps constructs. Dissociation constants are indicated where applicable. Error bars, 95% CI.