TABLE 2:
Microtubule dynamics parameters with full-length Msps transgenes.
| Events/minute | Velocity,b μm/min | |||||||
|---|---|---|---|---|---|---|---|---|
| Constructa | G → S | G→ P | S →G | S → P | P → G | P → S | Growth | Shortening |
| Control dsRNA (5, 2230) | 1.057 | 6.137 | 1.114 | 6.223 | 3.037 | 2.813 | 6.134 (±4.21, 3527) | 6.077 (±5.11, 2606) |
| Control dsRNA + FL-Msps (3, 545) | 1.286 | 6.415 | 1.149 | 6.107 | 2.446 | 3.219 | 6.568 p = 0.0029**c (±5.19, 1308) | 7.283 ****c (±6.24, 1322) |
| Msps dsRNA (3, 1008) | 1.161 | 6.871 p = 0.004**c | 0.740 p = 0.039*c | 6.728 p = 0.007*c | 1.930 p = 0.035*c | 4.057 p = 0.001**c | 6.406 (±4.62, 897) | 6.727 p = 0.017*c (±5.91, 1289) |
| Control dsRNA + FL-Msps (3, 545) | 1.286 | 6.415 | 1.149 | 6.107 | 2.446 | 3.219 | 6.568 (±5.19, 1308) | 7.283 ****c (±6.24, 1322) |
| Msps dsRNA + FL-Msps (3, 1219) | 1.313 | 5.842 p = 0.011*d | 1.633 p = 0.0096*d | 5.577 p = 0.018*d | 3.275 p = 0.0023**d | 2.971 | 7.021 p = 0.011*d (±5.59, 3431) | 8.415 ****d (±7.48, 2803) |
| Control dsRNA + Linker2 Mut (3, 473) | 1.633 | 6.257 | 1.573 | 5.930 | 2.886 | 2.594 | 7.591 ****d (±7.28, 1045) | 9.092 ****d (±8.71, 1030) |
| Msps dsRNA + Linker2 Mut (3, 509) | 1.202 | 6.050 | 1.534 | 5.635 | 2.813 | 3.949 | 7.585 ****d (±6.248, 1183) | 9.063 ****d (±8.228, 1105) |
| Control dsRNA + Linker2&4 Mut (3, 665) | 1.266 | 6.233 | 1.238 | 5.976 | 2.306 | 2.373 p = 0.0076*d | 7.334 p = 0.0007**d (±6.40, 1414) | 9.795 ****d (±8.81, 1366) |
| Msps dsRNA + Linker2&4 Mut (3, 421) | 1.019 | 5.658 p = 0.0089*d | 1.2010 | 5.513 p = 0.0315*d | 1.751 p = 0.0159*d | 2.113 p = 0.0016**d | 5.516 ****d (±6.45, 1110) | 7.329 (±7.89, 949) |
aNumbers in parentheses are the number of cells analyzed and the analyzed events for each condition, respectively.
bStandard deviations and number of measurements are indicated in parentheses below each value.
cValues statistically different from control dsRNA where p < 0.05.
dValues statistically different from control dsRNA + FL-Msps where p < 0.05.
*p < 0.05; **p < 0.005; ****p < 0.00005. Statistical significance for transition rates was determined using a two-tailed permutation test with 10,000 resamples. For growth and shrinkage rates, a two-sample t test was used to determine statistical significance.