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. 2011 Nov 15;22(22):4362–4372. doi: 10.1091/mbc.E11-04-0283

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© 2011 O'Leary et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).

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FIGURE 6: — Effect of proximal and distal VCD truncations on LAMP-2 colocalization and RFP-LC3 recruitment. Δct163-GFP (A) colocalized with LAMP-2–positive organelles and (B) recruited RFP-LC3 (A and B, arrowheads), similar to Δconst-GFP and full-length γA3-GFP. In contrast, Δct183-GFP partially colocalized with LAMP-2 organelles (A, arrowheads) and lacked RFP-LC3 recruitment activity (B, arrows). Removal of an additional six amino acids (Δct190-GFP) completely eliminated LAMP-2 colocalization, as well as RFP-LC3 recruitment (arrowheads). Bar, 10 μm.