TABLE 1:
Summary of MARCM experiments to study the function of vesicular trafficking during asymmetric division of larval NBs.
| Miranda localization | aPKC localization | ||||
|---|---|---|---|---|---|
| Gene | Allele | Normal | Mislocalized | Normal | Mislocalized |
| sec5 | sec5E10 | 18 | 0 | 18 | 0 |
| sec6 | sec6Ex15 | 23 | 0 | 23 | 0 |
| sec15 | sec151 | 14 | 0 | 14 | 0 |
| Rab5 | Rab52 | 18 | 0 | 18 | 0 |
| alpha-adaptin | ada3 | 20 | 0 | 20 | 0 |
| Hrs | HrsD28 | 21 | 0 | 21 | 0 |
| Vps25 | Vps25A3 | 14 | 0 | 14 | 0 |
| Sara | Sara12 | 15 | 0 | 15 | 0 |
| erupted (TSG101) | ept2 | No clones obtained | |||
| lgd | lgdd7 | 15 | 0 | 15 | 0 |
| aPKC | aPKCk06403 | 0 | 10 | Not detectable | Not detectable |
Numbers indicate in how many dividing NBs in MARCM clones of the indicated genotype Mira and aPKC were normal or mislocalized. All analyzed NBs were in late prophase to anaphase. “No clones obtained” indicates that under the chosen experimental conditions no clones containing NBs homozygous for the indicated null alleles were recovered.