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Different thermal regimes used for cell lysis
| Step no. | Parameters for thermal regimea:
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| I
|
II
|
III
|
IV
|
V
|
||||||
| H | T | H | T | H | T | H | T | H | T | |
| 1 | 80 | 1 | 80 | 1 | 80 | 1 | 95 | 1 | 98 | 5 |
| 2 | 8 | 0.5 | 4 | 0.5 | 4 | 0.5 | 4 | 0.5 | 4 | 1 |
| 3 | 95 | 5 | 95 | 8 | 95 | 5 | 95 | 5 | ||
| 4 | 8 | 1 | 4 | 1 | 4 | 1 | 4 | 1 | ||
| 5 | 95 | 3 | ||||||||
| 6 | 4 | 1 | ||||||||
H, heating-cooling temperature (°C); T, time (minutes).
