TABLE 3.

Oligonucleotide primers and conditions used for hsp-based PCRs for mycobacteria

Amplicon length (bp)	Direction and sequence of primer		PCR program
	Forward (5′-3′)	Reverse (5′-3′)
439	ACCAACGATGGTGTGTCCAT	CTTGTCGAACCGCATACCCT	Aa
228	CTGGTCAAGGAAGGTCTGCG	GATGACACCCTCGTTGCCAAC	Bb

^aProgram A consisted of 35 cycles of 94°C for 60 s, 60°C for 60 s, and 72°C for 60 s.

^bProgram B consisted of 30 cycles of 95°C for 15 s, 58°C for 15 s, and 72°C for 30 s.