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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: Methods Enzymol. 2010;475:27–59. doi: 10.1016/S0076-6879(10)75002-3

Figure 2.6. Super-resolution images of MreB in live C. crescentus cells.

Figure 2.6

(A–B) Images taken using standard PALM (C–D) images taken using time-lapse imaging to obtain higher labeling density using. (A) Image of MreB forming a midplane ring in a predivisional cell. (B) Banded MreB structure in a stalk cell. (C) Quasi-helical MreB structure at 40 nm resolution observed using time-lapse PALM. (D) Structure in panel (C) displayed without white-light image in order to highlight the continuity of the structure. (E) Time-lapse PALM image of MreB midplane ring in a predivisional cell. (Adapted and reproduced with permission from Biteen et al., 2008. Copyright 2008 Nature Publishing Group.)