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. Author manuscript; available in PMC: 2011 Nov 15.
Published in final edited form as: Methods Enzymol. 2010;475:27–59. doi: 10.1016/S0076-6879(10)75002-3

Figure 2.8. Single-molecule superlocalization using the DH-PSF.

Figure 2.8

(A) Image of a single DCDHF-V-PF4-azide molecule coming through the DH-PALM imaging system. (B) Histograms of 44 positions of the single molecule in (A) with standard deviations of 12.8 nm in x, 12.1 nm in y, and 19.5 nm in z. For these nonoptimized measurements, the average number of photons detected was 9300 with background noise of 48 photons/pixel and a pixel size of 160 nm. (Adapted and reproduced with permission from Pavani et al., 2009. Copyright 2009 National Academy of Sciences.)