TABLE 1.
Parameters used to genotype unknown HCV specimensa
Retention time (min) for the following amplicon: | Genotype | |
---|---|---|
153 bp | 45 bp | |
<3.5b | <5.0 | 2b |
3.5-4.5 | >5.3 | 3a |
4.2-4.5c | <5.0 | 2a |
4.4-5 | 5.0-5.3 | 1a |
>4.5 | 5.0-5.3 | 1b |
>4.7 | >5.3 | 4 |
Parameters are described for each amplicon separately but must both be present in the same sample for the sample to be allocated into a specific genotype.
The trace usually has a high absorbance and a shoulder.
The trace sometimes has a sharp spike associated with the trailing edge of the peak.