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. 2004 Jan;42(1):158–163. doi: 10.1128/JCM.42.1.158-163.2004

TABLE 1.

Parameters used to genotype unknown HCV specimensa

Retention time (min) for the following amplicon: Genotype
153 bp 45 bp
<3.5b <5.0 2b
3.5-4.5 >5.3 3a
4.2-4.5c <5.0 2a
4.4-5 5.0-5.3 1a
>4.5 5.0-5.3 1b
>4.7 >5.3 4
a

Parameters are described for each amplicon separately but must both be present in the same sample for the sample to be allocated into a specific genotype.

b

The trace usually has a high absorbance and a shoulder.

c

The trace sometimes has a sharp spike associated with the trailing edge of the peak.