Location of IS6110 upstream from phoP in the M. bovis B strain. (A) Schematic representation showing the position of IS6110 in the phoP promoter region. (B) Nucleotide sequence surrounding the second copy of IS6110. The IS6110 nucleotide sequence is boxed. The TGGC DRs flanking the IS6110 insertion site are shown in boldface characters. The ATG start codon of phoP is indicated by an arrow. The nucleotide sequences of primers BCG2A, BCG2B, phoBO4.1(T7), and ISA3 are underlined. ▾, transcription starting points tsp2 and tsp1 from pSO5 and pSO7 (tsp1*). tsp1*, tsp1 position in pSO5 wild-type M. tuberculosis. The nucleotide change (A for G) 46 bp upstream from the phoP ATG start codon of M. bovis with respect to M. tuberculosis H37Rv is indicated by an asterisk.