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The copy of IS6110 in the phoP promoter region is specific to B strains. Genomic DNA was PCR amplified with primers BCG2A and BCG2B (Fig. 2B). Lanes 3 and 13 to 15, B strains; lane 4, M. tuberculosis H37Rv; lanes 5 to 12, other MDR M. tuberculosis complex strains; lane 1, Gene Ruler 100-bp DNA Ladder Plus (MBI Fermentas, Vilnius, Lithuania); lane 2, λ/PstI. The presence of a 1,828-bp PCR product means that IS6110 was present in the promoter region of phoP in the analyzed strain.
