FIG. 4.

pSO5 and pSO7 mycobacterial plasmids. The phoP genes (including the promoter region) of M. tuberculosis and M. bovis B were amplified by PCR and ligated to pNBV1 that had been cut with XbaI to obtain pSO5 and pSO7, respectively. pSO5 contained a 2,046-bp M. tuberculosis fragment containing a 1-kb region upstream from the ATG start codon plus the entire nucleotide sequence of phoP (30). pSO7 contained the equivalent region from the B strain (see text for more details). hyg, hygromycin-resistant gene; ori myc, mycobacterial origin of replication; ori, E. coli origin of replication.