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Expression of M. avium subsp. paratuberculosis six-His tag clones in E. coli. An immunoblot containing IPTG-induced E. coli BL21(DE3) lysates was probed with a monoclonal antibody that detects the polyhistidine tag. Size standards (in kilodaltons) are indicated on the left. Lanes: 1, protein size standards; 2, gene 254; 3, gene 159; 4, gene 56; 5, gene 38; 6, gene 228; 7, E. coli BL21(DE3) cells.
