Figure 1. Western blot to demonstrate the depletion of MCM7 from S2 cells by dsRNA interference.

The levels of MCM7 protein were determined 3, 5 and 7 days after the addition of dsRNA in control cells and cells treated with dsRNA against MCM7 as shown. Loading was checked by comparison with Ponceau red staining of the blot before development (data not shown). The cross-reacting but unrelated band (*) also provides an indication that loading between the fractions is comparable.